Plasmid_Backbone

Part:BBa_J18968:Design

Designed by: Sean Sleight   Group: Affiliates   (2013-06-18)


pSS3C1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1187
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 178
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1273
    Illegal AgeI site found at 1596
    Illegal AgeI site found at 4413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2301
    Illegal SapI site found at 4395


Design Notes

Inserted luxR and araC to be constitutively expressed in the reverse direction on the vector. Removed restriction sites in prefix and suffix to improve assembly success rate.


Source

Modified pGA3K3 from 2011 University of Washington iGEM team.


References